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We chose two nuclear genes SQD1 (Region 1) and ApPEFP_C (Region 2), redesigning (now with suffix _ptaq) the forward ApPEFP_C and SQD1 reverse, based on Pteridium sequences from a study of the transcriptome (Der et al. 2011).
Although the total access increases, some populations show a worse composite measure, which indicates that they are traveling shorter distances but experiencing higher congestion (or the reverse) based on new network dynamics.
One COI partial region was amplified with primers SacCOIF and SacCOIR [ 33] and the trnW-L partial region from the 3′ end of the trnW gene to the 5′end of the trnL was amplified with primers 5′-GGTTCAAGTCCCTCTCTTTCTGT-3′ (trnW-LBF, forward) and 5′-AACCTAAACCCAGCGTGTAT-3′ (trnW-LBR, reverse) based on the mitochondrial genome sequence of S. japonica [ 5].
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False confessions figured in about twenty per cent of the two hundred sixty-six convictions that have been reversed based on DNA evidence, according to the Innocence Project's count.
Although investment levels have slowly risen in Honduras since 2002, this trend is likely to be reversed based on the political change that occurred in the country earlier this year.
Both studies would demonstrate that differentiation from chloroplast to amyloplast could be reversed based on physiological changes.
MiRNAs with complete or partial overlaps with genes were classified as intragenic or intragenic-reverse, based on relative orientation.
The following primers were designed as forward and reverse primers based on the female, male specific tags and α-tubulin gene (endogenous control): AMP-activated kinase F: 5'-TGCTAGTGGTAAATGGGGTGT-3', R: 5'-TTCATTGTACCATTGGATATTTTCAT-3'.
Following sequencing, we assembled the sequences for each exon using its forward and reverse sequences, based on the reference exon sequence from the published human and mouse genomes.
Specific forward or reverse primers based on the nucleotide sequences of the elements TremA and TremB were used with oligo dT) to amplify sequences by PCR.
QuEST [ 28] combines forward tags and reverse tags based on a kernel-density-estimation approach and a "peak-shift" strategy, and then identifies significant peaks and provides peak summits an estimate for TFBSs.
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