The other two reactions were performed using either forward or reverse primers alone.
In strand-specific RT-PCR, reverse transcription is primed with a gene specific forward or reverse primer alone, thereby generating cDNA of specifically the antisense or sense strand of the targeted region respectively.
But frankly, we don't even have a plan to contain Iran, let alone reverse its regional gains.
In those circumstances, it was going to take a different brand of local government simply to arrest that decline, let alone reverse it.
It may take much longer to restore a minimum of trust to Haitian political life, let alone reverse the country's economic ruin.
Iraq's leaders, struggling first with the post-Saddam Hussein strife and now with a political impasse that has delayed the formation of a new government, have so far been unable to do much to avert the catastrophe unfolding here, let alone reverse it.
"But some companies have begun to struggle, and it is difficult to see just how they can reverse their fortune alone".
Drug companies and researchers are seeking ways to restore, protect, or extend a person's telomeres, but the jury is still out on whether such interventions can slow the symptoms of aging, let alone reverse them.
Therefore, if we judge as we have that Yahoo has managed to execute its mobile strategy of using growing mobile usership to expand its sold ads base, we can also come to the conclusion that it will have to reach a scale far greater than its current tally to stop, let alone reverse, its current revenue declines.
That's why she has no program at all to halt, let alone reverse, runaway inequality.
