Sentence examples for reverse alignment from inspiring English sources
The conjunct contigs were selected from a combination of the aligned contigs of forward and reverse alignment results.
The templates are ranked based on the difference score between the raw alignment score and the reverse alignment raw score in which the alignment is made with the reversed query sequence [36].
We found that ranking based on the difference score between the raw alignment score and the reverse alignment raw score works well only if there is some structural similarity between the top-two ranked models (i.e. a significant structural cluster detected).
Based on the feature of the stem-loop structure, a command line BLAST was performed to align each unique miRNA sequence with the sequences of the transcriptomic database, aligning forward and reverse strands separately by setting the parameter –S (1 for forward, 2 for reverse alignment).
The generated forward and reverse alignment files were merged together using SAMtools [ 31].
DELLY computes two scoring matrices S F and S R, one for the forward and one for the reverse alignment using the Gotoh algorithm with affine gap penalties from the SeqAn library (Döring et al., 2008; Rausch et al., 2008).
The red and blue lines represent direct and reverse alignments, respectively.
Forward to forward alignments are show in normal text, forward to reverse alignments are shown in italics.
If a reporter has alignments in the reverse direction, but also has at least one high-quality forward alignments to a RefSeq RNA whose status is more definite than "model", then the reverse alignments are ignored and the reporter remains under consideration for being fully valid or RefSeq RNA valid.
Alignments were manually adjusted and DNA sequences reverse aligned to correspond with protein alignments.
Thus, for totally unambiguous cases, reversing residue order prior to alignment should yield an exact reversed alignment of that obtained by using the unreversed sequences.
