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This hypothesis was supported by the reversal expression of Usp9x, which is X-linked and expressed in both male and female embryonic germ cells, but turned off in male germ cells after birth [ 31].
Although derived from the same gene locus and transcribed by the same transcription factors, the 5p arm and 3p arm miRNA might have reversal expression preference.
In this study, we show that although the 5p arm and 3p arm miRNAs are derived from the same gene locus and transcribed by the same transcription factors, they have significant reversal expression preference between two tissues.
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As a future direction, it would be equally important to investigate the length of time the gene expressions persist during a medication-free follow-up period and whether the reversal of expression to premedication state would lead to relapse.
Particularly, delayed reversal of expression profiles was observed for CxCR4, TNFAIP3, SOCS2, NR4A2, and POLR2J (Figure 3).
This also induced reversal of expression of key cholesterol metabolic genes (data not shown).
These data show that the growth-arrested phenotype of Rasless cells cannot be corrected by reversal of expression levels of Sca1 alone.
The importance of the HGF pathway was further documented in our system by blocking HGF signaling with an antibody, resulting in reversal of expression and morphologic phenotypes.
In other words, we investigated whether the 5p arm and 3p arm miRNA derived from the same pre-miRNA have reversal tissue expression preference without considering miRBase annotation.
We used demethylating agents including 5-Aza-2'-deoxycytidine (5-Aza-2'-dC) and the histone deacetylase (HDAC) inhibitor Trichostatin A, to assess reversal of expression of Cyclin D2 in astrocytic cell lines wherein the gene is not naturally expressed.
Furthermore, the discovery phase of this study [ 3] included a longitudinal analysis of candidate biomarkers to demonstrate reversal of expression patterns over time, thereby verifying the pathophysiological relevance of these proteins.
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