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Epigenomic maps reveal enhancer elements unique to NRs relative to 2D systems.
From early experiments that defined and mapped the first enhancers to recent enhancer models of human disease, functional experiments in the mouse have played a central role in revealing enhancer biology.
Independent enhancer-reporter transgene analysis of nub-8 through nub-13 conserved sequence clusters (CSCs) reveal enhancer function in the third instar larvae during larval CNS development.
Kaaij, L. T. et al. DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus.
Integrating eQTL, mRNA co-expression, and Hi-C data analysis, we developed a computational method to infer causal enhancer-gene interactions, revealing enhancers of clinically actionable genes.
We first evaluated the GR binding and enhancer properties of sites belonging to each mode of nucleosome remodeling.
Certain expression pattern components, seen with more reporter fusion genes than expected based on other expression pattern data and which may result from cryptic enhancers in the cloning vector only revealed according to properties of the particular promoter being assayed, do need to be treated with scepticism but are readily recognised in large scale studies.
Experiments on the mouse enhancers reveal that enhancers active in different tissues can be separated by pairwise comparison using N2.
Comparison of interactions between cell types revealed that enhancer-promoter interactions were highly cell-type-specific.
In total, this screen revealed three enhancers and one suppressor of HSC-derived reconstitution.
Yaworsky, P. J. & Kappen, C. Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene.
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