Global gene expression studies comparing subcutaneous and visceral adipose tissue have revealed depot-specific expression of homeobox genes [33], [34].
Morphometry analysis of inguinal adipose depot revealed a significant decrease in adipocytes size in CNX-012-570 CNX-012-570 CNX-012-570ared treatedHFD control animals.
Gene expression of hSAA in three adipose tissue depots revealed different levels of expression: BAT>rWAT>eWAT.
Previous data revealed accumulating fat depots in skeletal muscle in older subjects, leading to metabolic dysfunction through lipotoxicity and to insulin resistance (Petersen et al., 2003; Zoico et al., 2010).
Histological examination of high fat diet fed mice after 16 weeks of treatment with TNP-470 revealed that the depots of subcutaneous, perigonadal, and omental fat were dramatically decreased compared with control mice.
To assess fibrosis Masson's trichrome staining was performed, which revealed the epididymal WAT depot of the HFD group contained very pronounced trichrome-positive "streaks" interspersed among the adipocytes at 24 weeks.
It was later revealed that this first depot-laying season, and its attendant hardships, had been unnecessary.
By dividing the epididymal adipose tissue into a proximal and a distal section we revealed functional zonation in the depot, whereby proximal EWAT exhibited reduced synthesis and accumulation of fatty acid as compared to the two other adipose locations.
Interesting insights into the nature of WAT depot-dependent gene expression were, however, revealed in their further analysis of WAT depot-dependent expression of 12 embryonic development and pattern specification genes [ 19].
Recently there has been renewed interest in the role of BAT in humans since fluorodeoxyglucose positron emission tomography has revealed the presence of BAT depots in adult humans [ 2].
Analysis of epigonadal WAT, brown adipose tissue, and subcutaneous fat from DKO mice revealed a severe reduction in fat depots and adipocyte cell size, compared with control and single-mutant mice (Fig. 2A,B and data not shown).
