Exact(5)
In support of this idea, Jones and colleagues [2] suggest that the first returned BLAST matching hit is a fairly good strategy for annotating novel sequences, arguing that new methods should at least be able to outperform this approach.
For the "reference" dataset (Table 1), 61,340 sequences returned BLAST hits against Metazoa - 12% of those were specifically poriferan; another 15% were to other Metazoa.
At a minimum of 50 out of 69 bp (72%) sequence match, 52 of the 59 outlier markers returned BLAST matches to the 11 main scaffolds of the E. grandis genome.
Three novel bat miRNAs were located within this cluster, including one (pal-can-276) that returned BLAST hits to miR-541 but had three mismatches relative to other vertebrates, including two unique differences in the seed region.
Of all P. larvae phage putative proteins, 86% had a BLAST hit with an E-value less than 1 × 10-4 (see Table 3), yet only 48% of the proteins returned BLAST hits listing a function.
Similar(55)
EnsemblPlants allows users to blast query sequence to up to 25 species, and then returns blast report in text format.
When the probe coordinates were found to overlap with more than one gene or when blasting against the Ensembl bovine database returned multiple blast hits with a difference in nucleotide coverage between the first and second best hit of <10%, the probe was discarded.
One of these (pal-can-303) returned a BLAST hit to miR-506, while the other two returned only low-quality BLAST hits (however these included miR-465, which is also located on the X chromosome in humans and a member of an equivalent miRNA cluster in rodents).
One BLAST run against Uniprot takes 1 or 2 min. The default number of hits returned by BLAST is 250.
England were terrorised by Mitchell Johnson, who removed Alastair Cook – looking desperately tired – with his fourth ball and returned to blast out Jos Buttler and Moeen Ali in his first over after tea.
Two weeks later, she bombarded the industrial complex at Hamamatsu before she returned to blast Kamaishi on 9 August.
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