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Should you grow weary of in-air mousery, you may, of course, return the mouse to the surface of your desk and use it like an ordinary human interface device.
Return the mouse to it's normal position on the mouse pad.
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The guillotine door was then removed and the experimenter returned the mouse to the start arm of the T-maze for the choice run of the trial.
Once the mouse found its experimental cage again behind the exit tube, the investigator entered the room and returned the mouse, inside its cage, to the adjacent room where the animal was returned to its home cage.
Once the researchers had instilled the fear memory, they repeatedly returned the mice to the cage without the shock, either 24 hours or 30 days later.
Afterwards, they returned the mice to the lab, let ones within each group interbreed, and took some cells from the tails of both the mice and their offspring.
After returning the mice to their cages, over the course of 6 weeks the researchers placed the rodents back in the box but did not repeat the shocks.
Contextual fear conditioning is evaluated by returning the mice to the environment in which they were exposed to the electrical shock.
Heparinase III was injected into the eyes at P12 immediately after returning the mice to room air (at the end of 5 days' exposure to 80% oxygen).
On day 3 (testing day), 24 hours after conditioning, place preference was measured by returning the mice to the place conditioning environment for 5 min. The time spent (sec) in the air puff chamber and the safe chamber was measured and the proportions of time spent by each mouse in the air-puff chamber were calculated and used as the place preference index.
Next, we studied VEGF-A levels in the ocular fluid at P14, two days after the administration of heparinase III at P12 (immediately after returning the mice to room air), where elevated VEGF-A levels in OIR was not affected by heparinase III treatment, i.e., no difference was detected compared with PBS-treated eyes (Figure 3B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com