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Fig. 3 Scanning electron micrographs at ×1600 magnification of A as received superelastic NiTi wires, B retrieved superelastic NiTi wires, C as received heat-activated NiTi wires, and D retrieved heat-activated NiTi wires.

The antigens were retrieved by heat treatment in pH 8.0 Tris-EDTA buffer (Cell-Conditioning Solution, Ventana) at 95°C for 30 min for WNT5A.

Epitopes were retrieved by heat (900 W microwave, 10 min) in 10 mM citrate buffer (pH 6.0) and endogenous peroxidase activity was blocked using 0.3%H2O210 (10 min).

Antigens were retrieved by heat inactivation in citrate buffer, followed by incubation with mouse anti-human CD68 (1 400, DakoCytomation, Glostrup, Denmark).

The staining retrieved by heat pretreatment could thus be due to detection of oligomeric Aβ fibrils by the OC antibody, which is consistent with two previous studies suggesting that Aβ oligomerization starts within neurons [ 59, 64].

With the high potentials of its efficient heat retrieving unit to effectively use low temperature solar energy, the investigation of the heating and cooling applications of the system has advanced within the past decade.

In total 3- μm-thick sections were deparaffinised and pretreated for antigenic heat retrieving 1 h at 98°C with 10 m M citrate buffer pH 6.

After deparaffinization with xylene and rehydration with serial gradient ethanol, the antigen was retrieved by heating the slides in 10 mM of citrate buffer (pH 6.0) for 6 min in a microwave.

Epitopes were retrieved by heat-treatment in citrate buffer (DAKO).

Antigens were retrieved by heating the tissue sections in citrate buffer (pH 6.0) by microwaves.

Antigens were retrieved by heating the sections in 0.01 M citrate buffer (pH 6.0).