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The medium was replaced on day 1 and 3; and the retrieved medium was stored at −20 °C until progesterone assay.
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On the other hand, the results obtained with the P1B-HTK system confirm the difficulty in retrieving medium-length terms for the phone-based system submitted to the evaluation when the term properties produce a more difficult term.
However, recent research has demonstrated that coherent crop specific biophysical variables such as green area index (GAI) can be retrieved from medium spatial resolution imagery such as MODIS even when the size of the fields is close to the size of the pixels (close to 250 m).
For the assay, an aliquot of medium retrieved from cultures (50 μl DMEM or D10 in the presence and absence of ARPE-19 cells) were mixed with 500 μl of complete FOX reagent.
To illustrate the performances of these algorithms, the thermal conductivity, absorption coefficient and scattering coefficient of the 1-D homogeneous semi-transparent medium were retrieved for four test cases.
At the indicated times equal aliquots of medium were retrieved and released 59Fe was quantified in a γ-counter.
A 50 µl aliquot of the medium was retrieved at each time point and counted in a γ-counter.
The oocytes were retrieved in the same medium without heparin using a stereo microscope.
After incubation for 24 h, the culture medium was retrieved and viral supernatants were added to each well.
Only one of the three implanted pellets of the second switch condition where β-glycerophosphate was added to the chondrogenic medium, was retrieved.
After addition of H2O2 (pulse delivery) or GOx (to initiate continuous H2O2 generation), aliquots of culture medium were retrieved at intervals to determine H2O2 levels, and cells were harvested after 24 h (treatment-1 and GOx) or 2 h (treatment-2) to perform cytotoxicity assays by the methods described below.
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