Vesicles are filtrated using a fiberglass Milipore filter (< 1μm pore size) and eluted with pure methanol to retrieve substrate, which is later analysed.
The retrieved substrates were then rinsed with fresh TFE, vacuum-dried, and subjected to XPS analysis.
In this article, we introduce METSP, a maximum-entropy classifier devoted to retrieve transporter-substrate pairs (TSPs) from semistructured text.
This model has been used as a query for virtual screening to retrieve potential new substrates from the small-molecule, 'lead-like' databases of MOE.
O. piceae has to retrieve nutrients from a nutrient-poor substrate that typically contains very little nitrogen and relatively low levels of host defence chemicals.
P. putida-specific primary and review literature and books were used to retrieve information about every network reaction: i) substrate specificity, ii) coenzyme specificity, iii) reaction directionality, iv) enzyme and reaction localization, and v) gene- protein- reaction (GPR) association.
Users may search strains within a range of values for one or several properties, including cultivation temperature, substrate, or application, before retrieving the retrieve corresponding results.
Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues.
To determine the level of site conservation of the NatB substrate recognition motifs, MD, ME and MN, the orthologous sequences of 59 NatB substrates were retrieved from EGGNOG v2.0 [ 37].
Protein sequences of described CAPN3 substrates were retrieved from Uniprot (release 14.6) (http://www.expasy.org/sprot/) and aligned in TEIRESIAS (http://cbcsrv.watson.ibm.com/Tspd.html).html
