The samples and surrounding tissue were retrieved, fixed with 10 vol% formalin solution, and embedded in paraffin.
At 4 weeks after transplantation, the diffusion chambers were retrieved, fixed in paraformaldehyde, and embedded in Tissue-Tek OCT™ compound or paraffin.
After 2, 4, and 8 weeks, the mice were euthanized and the implants were retrieved, fixed in buffered formalin (10%) for 24 h, and decalcified in 50 mM EDTA for 1 week.
As it was, the company had to remotely rip out, retrieve and fix dozens of complex and heavy pieces of equipment lying on the sea floor, some weighing more than 400 tons.
Although the presented estimates are retrieved from fixed effect specifications, they can be replicated using differences-in-differences specifications with a time-constant control variable for the treatment group using OLS or random effects estimation.
The livers were immediately retrieved and fixed in 10% neutral buffered formalin for 24 hours or kept freshly frozen.
After sacrifice, bilateral tibias and lumbar vertebrae (L2 and L3) were retrieved and fixed in 70% ethanol for 7 days.
After CT analysis, both tibias of each dog were retrieved and fixed using 10% neutral buffered formalin for 7 days.
Tumours were retrieved and fixed following the final PD170374 treatment and sections stained for Ki-67 and TUNEL to assess effects on proliferation and apoptosis respectively.
After 11 days, the sponges were retrieved and fixed in 10% formalin, then subjected to flow cytometry or immunohistochemistry for cell markers as deemed necessary.
After 1 day (N=1) or 21 days (N=4) post-surgery, the rabbits were euthanized under ketamine-xylazine anesthesia by IV injection of sodium pentobarbital and the femoral ends were retrieved and fixed in 4% paraformaldehyde/100mM cacodylate, pH 7.4.
