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For the P0 group, the centrifuge was briefly (<15 min) stopped three times per day at eight hr intervals to retrieve dams that were undergoing labor contractions or birth.
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E15.5 fetuses retrieved from dams periconceptionally fed with the HC diet weighed similar to fetuses from chow-fed mice [382 ± 22 mg (n = 11 fetuses) versus 393 ± 22 mg (n = 24 fetuses), resp., P = 0.58 Student's t-test].
Interestingly, 67% of dams that failed to retrieve pups during the pup retrieval test (compared to only 6% of dams that successfully retrieved pups) scattered their pups in the maternal behavior assay (p < 0.001), suggesting dams that scattered pups were unlikely to retrieve their pups.
However, dnFGFR dams were more likely to leave their pups scattered and took significantly longer to retrieve each pup compared to control dams.
There was a cut-off after 300 seconds if the dam did not retrieve her pups.
The barrier was then removed and each dam was scored for the total number of pups retrieved in 10 min and the latency to retrieve the first and all of the pups back to the nest (39).
Fast retrieve or slow retrieve?
"Retrieve your metals".
The dam was then returned to the center of the home cage and the latencies to sniff one of the pups, retrieve each of the three pups, and crouch over them for longer than a minute were recorded.
Retrieve the crafted dispenser.
Retrieve a bowl.
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CEO of Professional Science Editing for Scientists @ prosciediting.com