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Antigen retrieval was made by heating for 1 min in Tris/EDTA buffer in a pressure cooker.
Antigenic retrieval was made by section immersion in citrate buffer, kept at high temperature (100°C) for 45 minutes.
After the quenching step, antigen retrieval was made with citrate buffer (pH 6.0) in pressure cooker for 30 minutes and then sections were kept at room temperature for 20 30 minutes.
Sections were deparaffinised in xylene baths then rehydrated in graded alcohols to water antigen retrieval was made by HIER method using a steamer (90°C for 30 minutes), in high pH Dako retrieval solution; endogenous peroxidase activity was quenched by 0.3 H2O2 at room temperature, 10 minutes.
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Kroon's own clinic, Queensland Fertility Group, has protocols in place should a request for posthumous sperm retrieval be made.
In a similar way as applied to the dust model parameters, sensitivity analysis for water vapor retrieval is made by changing the value of c w between 0.476 and 4.76 mg/correspondingnding to 100 and 1000 ppm, respectively).
If a 'borrowing' is in progress, the model first subtracts 1 from the upper term before the initial retrieval is made.
A demo version of the search engine SCAIView, including the biomarker retrieval, is made available to the public through http://www.scaiview.com/scaiview-academia.html.html
While, for example Douville et al. [7] did compare processing of familiar vs. unfamiliar persons, whose names were provided to participants and no memory retrieval was required, however, no comparison was made with common name processing in this fMRI study.
Further linkage to the pathological registry was made for the retrieval of histopathological samples for re-review and laboratory analysis.
Egg retrieval was performed 36 hours later, and insemination was made by conventional IVF or ICSI depending on semen parameters.
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