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Antigen retrieval was completed in boiling sodium citrate buffer (PH 6.0) for 10 min.
Slides were deparaffinized using xylenes and a graded ethanol series and antigen retrieval was completed using citrate buffer (pH 6) and steam heat.
Antigen retrieval was completed by cooking tissue sections for 3 min with citrate buffer (pH 6.0) for cleaved Caspase-3 and Skp2, and in EDTA buffer (pH 8.0) for Ki-67.
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Most of the properties however have to be derived by the user after the data retrieval is complete by using additional tools.
Initially, a Medical Literature Analysis and Retrieval System Online (MEDLINE) search through PubMed was completed for a general overview of the literature.
The study, including the retrieval of data from the publicly available electronic databases, was completed by June 2010.
A transfer test was completed at the end of day 2 and consisted of two runs (encoding and retrieval).
To ensure the CA1-specificity, the age of iCA1-KO mice by the time the retrieval experiments were completed was no larger than 9 weeks [13], [17].
Once the above offline procedure has been completed, the retrieval engine is functional.
Behavioral observations were completed by a pup retrieval test [39], [40].
Of the 120 questionnaires distributed, 110 were completed and returned, giving a 91.7 % retrieval rate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com