Exact(21)
Application to much-needed verb retrieval treatment is also considered.
For diagnostics using FFPE specimens, the fixation time should not be too long and protease should be used for the antigen retrieval treatment.
Sections were subjected to antigen retrieval treatment using the Antigen Retrival kit (BioGenex Inc ., according to the manufacturer's protocol.
Formalin-fixed sections (human section) were, then, treated with target retrieval solution (DAKO, Carpinteria, CA) for 10 min in steamer; no target retrieval treatment for zinc-based Beckstead's solution-fixed mouse tissues.
Slides were rinsed with PBS prior to antigen retrieval treatment with 10 µg/ml proteinase K (Roche) in pre-warmed buffer containing 1 M Tris and 0.5 M EDTA (pH 8) at 37°C for 15 min. The treated slides were extensively washed with PBS prior to immunofluorescence staining.
Antigen retrieval treatment prior to staining was omitted to allow clearer visualization of extranuclear HMGB1.
Similar(39)
In Experiment 2, using conventional formalin-fixed paraffin-embedded (FFPE) procedures, the effects of different fixation times and antigen retrieval treatments were assessed.
The marker panel is summarized in table I, with results from various antigen retrieval treatments tested.
For immunohistological staining, tissue slices were subjected to different antigen retrieval treatments.
Taken together, these data reveal that antigen retrieval treatments that could potentially denature SOD1 produce C4F6 immunoreactivity for every SOD1 variant tested, including over-expressed WT hSOD1.
Identification of cell types in bovine tissue sections is complicated by the limited availability of anti-bovine antibodies, and by antigen retrieval treatments required for formalin-fixed tissue samples.
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