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Exact(6)
By using isogeometric analysis, the exact geometry is used in both the structural and aerodynamic models, preventing any additional computational effort for meshing and geometry retrieval steps and avoiding the introduction of geometrical errors due to the discretisation of the geometry.
Tissue sections were taken through deparaffinisation and target retrieval steps using an automated PT Link (Dako, Glostrup, Denmark).
The antibodies and antigen retrieval steps used for immunofluorescence and immunohistochemistry are summarized in supplementary material Table S3.
After sectioning, rehydration and antigen retrieval steps, von Willebrand's factor antibody (1 : 800, Abcam, Cambridge, UK) was applied to the section for 1 h at RT, followed by a goat polyclonal secondary antibody conjugated to FITC (30 min at RT).
After sectioning, rehydration and antigen retrieval steps, von Willebrand's antibody (1 : 800, Abcam, Cambridge, UK) was applied to the section for 1 h at RT, followed by a goat polyclonal secondary antibody conjugated to FITC (30 min at RT).
Immunohistochemical studies were performed automatically in the Bond Max equipment (Leica Microsystems Inc .. Antigen retrieval steps were performed in Bond-Epitope Retrieval Solution 1 (Leica Microsystems, Germany) with TAGLN antibody (1 500, Abcam ab1416) at 100 °C.
Similar(54)
Antigen epitopes shielded by formaldehyde cross-linked lysine side chains were retrieved in a heat-mediated antigen retrieval step using Target Retrieval Solution (Dako), according to manufacturers' protocol.
An antigen retrieval step was performed with R-Universal buffer in the 2100 antigen retriever for a single heat pressure cycle (Aptum Biologics).
Currently, linear formation of Local-Global method is commonly used in the retrieval step of CBR.
In this paper we try to improve the retrieval step for case based reasoning for preliminary design.
Dewaxed and rehydrated slides were subjected to antigen retrieval step for 20 minutes at 99°C using a high pH antigen retrieval solution.
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