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The sections were treated with different antigen retrieval solutions (Citra Plus (BioGenex, San Ramon, CA, USA), pH 6.03; AR-10 Solution (BioGenex, San Ramon, CA, USA), pH 10.7; Tris buffer, pH 7.3 as well as TUF solution, pH 5.7 (Kreatech, Amsterdam, Netherlands)) in a microwave oven, according to the manufacturer's instructions (see also Table 2).
In order to facilitate the search for IPs in large IP assets, e.g. virtual marketplaces, we have developed the IP Broker tool suite that facilitates the development of IP retrieval solutions by making use of Case-based Reasoning technology.
We demonstrate that certain shortcomings of existing content-based image retrieval solutions can be addressed by implementing a biologically motivated, unsupervised way of grouping together images whose salient regions of interest (ROIs) are perceptually similar regardless of the visual contents of other (less relevant) parts of the image.
All slides were processed with an automated staining system, the Leica Bond III autostainer (Leica Biosystems) used according to the manufacturer's protocol and with the manufacturer's retrieval solutions.
A microwave oven and pressure cooker designated for antigen retrieval, along with retrieval solutions, were purchased from Biocare Medical (Walnut Creek, CA).
After cooling retrieval solutions for 25 minutes at room temperature, the slides were treated for 5 minutes with Blocker of Endogenous Peroxidase (DAKO).
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Tissue sections were deparaffinized and antigen was retrieved by antigen retrieval solution.
Antigens were retrieved using the Dako Target Retrieval solution in conjunction with steam.
To stain for F4/80-positivity, antigens were retrieved using the Dako target retrieval solution (Dako, Carpinteria, CA).
Antigen retrieval was performed on rehydrated equatorial tissue sections using an EZ Retriever System with CitraPlus Antigen Retrieval Solution (BioGenex, San Ramon, CA, USA).
The antigen was retrieved using a steamer in epitope retrieval solution (IHC WORLD, Woodstock, MD).
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