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Tissue sections were deparaffinized and antigen was retrieved by antigen retrieval solution.
Antigens were retrieved using the Dako Target Retrieval solution in conjunction with steam.
To stain for F4/80-positivity, antigens were retrieved using the Dako target retrieval solution (Dako, Carpinteria, CA).
The antigen was retrieved using a steamer in epitope retrieval solution (IHC WORLD, Woodstock, MD).
Weng et al. [35] propose a two-level retrieval solution.
Briefly, sections were deparaffinized and treated with Target Retrieval Solution (Dako) according to the manufacturer's protocol.
Epitope retrieval was induced by microwave heating using 0.01 M citrate retrieval solution (pH 6.0).
Slides were then microwaved in antigen retrieval solution to remove the remaining antibodies.
For antigen retrieval, slides were pre-treated with the Bond™ Epitope Retrieval Solution 2 (Vision BioSystems).
Antigen retrieval was performed for 2 minutes in preheated target retrieval solution (Dako) using a pressure cooker.
Antigen retrieval was performed for 90 s in preheated target retrieval solution (Dako) using a pressure cooker.
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