After incubation in citrate buffer and boiling for heat-induced antigen retrieval, samples were blocked with goat sera and subsequently incubated with primary anti-nitrotyrosine (Millipore, Schwalbach, Germany) or anti-CSE antibodies (Abnova, Taipei City, Taiwan).
For antigen retrieval, samples were microwaved for a total of 20 minutes in a citrate buffer (pH = 6).
For antigen retrieval, samples were boiled for 9 min in 10 mM sodium citrate buffer (pH 6.0) and cooled for 5 10 min in water.
For antigen retrieval, samples were incubated 40 min at 100°C in a citrate buffer at pH 6. Endogenous peroxidase activity was blocked for 15 min with 0.3% hydrogen peroxide.
For antigen retrieval, samples were incubated with 0.1 M citrate buffer (pH 6.0) and heated in a pressure cooker for 8 min. After rinsing in TBS, the slides were incubated with 3% normal donkey serum (NDS) in TBS for 20 min to prevent non-specific binding of the first antibody.
Here, we design an experiment on PEB to simulate the case described above: Represent the models with BWs method and save the descriptors for the following usage as block 1 and 2 in Figure 2. When performing the Partial-to-Global Retrieval, the sampled oriented-basis points are grouped into regions according to their geometric positions first.
> -wrap-foot> Retrieval of samples with similar annotation: We applied the proposed approach and a number of alternatives for retrieval of similar samples from the same dataset and evaluated by how many of the retrieved samples had the same annotation: class label, disease state or body site.
Because both biological and genetic samples are collected from many of the studies, a data management system for the cataloguing and retrieval of samples was developed.
At all retrieval times, samples maintained shape and contained regions of distinct tissue formation.
For better comparative evaluation, multiple queries have been designed to efficiently address all types of experiments and platforms, and allow for retrieval of sample-related information, such as cell line, disease state and clinical aspects.
Retrieval of samples is faster if stored in "box-area" rather than "box-brain", for most requests include the same areas from many source brains.
