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An antigen retrieval method was used for the detection of PCNA.
For p-AKT (ser473; Cell Signaling, 1/50 dilution) and p-ERK1/2 (Cell Signaling, 1 100 dilution), citrate buffer and microwave antigen retrieval method was used.
Preparations were pretreated with citrate buffer (0.01 M citric acid pH 6.0), and a heat-based antigen retrieval method was used prior to incubations.
Heat-induced epitope retrieval in Tris/EDTA pH 9.0 buffer was used for CK5/6, c-KIT and Ki-67 staining, while proteinase K enzymatic retrieval method was used for EGFR staining.
In the case of SCIAMACHY, a 2-D tomographic retrieval method was used to obtain vertical profiles of Mg and Mg+ between 50 and 150 km, with a vertical resolution of 3.3 km.
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Since diffraction effects cannot be ignored in the optical regime, an iterative phase retrieval method is used to calculate the phase coded screen.
A cross-lingual information retrieval method is used to aid an LM in different language in [3].
Despite their presence in the normal mature adult rodent brain, staining for many basement membrane antigens including laminin and collagen IV occurs only inconsistently in standard perfusion-fixed tissues unless a proteolytic antigen retrieval method is used [ 14].
Briefly, the human tissue were co-stained for p63 and VDR with two different antigen-retrieval processes, a heat base antigen-retrieval method was used for p63 followed by an acid antigen-retrieval processes for VDR and finally neutralization with 0.1 M Borate Buffer pH8.5.
Two common retrieval methods are used for retrieving the most relevant MeSH documents, one based on language models (described in the online Supplementary Material) and the other using a vector-based representation, which are described below.
Antigen retrieval methods were used (Table 2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com