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Briefly, antigen retrieval for all epitopes was carried out using heat treatment under pressure in a microwave oven for 5 min in citrate buffer (82 ml 0.01 M sodium citrate: 18 ml 0.01 M citric acid) pH 6.0.
All staining was performed using a Dako autostainer following antigen retrieval for all antibodies except for Ki-67, which was performed on a Leica (Wetzlar, Germany)/Bond Max system using ER2 (high pH antigen retrieval).
Immunostaining was performed using BenchMark XT-Ventana Medical Systems with kit DABMAP (using streptavidin/biotin system) for CD31 or kit OMNIMAP (system "biotin-free" using multimer technology) for KI67 and VEGF with antigen retrieval for all (citrate buffer pH 6.0; Tris/Borate/EDTA pH 8.0).
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The rate of the optimal cloud retrievals for all trials is also computed for each cloud state.
To calculate the pooled ROC n score, merge the retrieval lists for all sample queries into a 'pooled retrieval list', and sort the pooled list on the E-value.
It includes four large desks staffed by as many as six librarians, and a busy retrieval point for all requested materials.
SIMPLE srf-SCD and srf-CEDD 512, in particular, achieve an almost perfect retrieval score for all evaluation metrics.
SQL Server 2005 is the relational database management system that provides the data storage, indexing, and retrieval functionality for all data stored on MOSS 2007.
Retrieval time for all memories produced by each participant (up to 15 memories) was averaged.
The overall average retrieval time for all the test queries in all situations was measured at 2.16 s/query.
Citric acid buffer, pH 6.0 (10 mM) was used as standard microwave-based antigen retrieval method for all proteins except for pACC.
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