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This movie shows a pup retrieval assay with a wild-type mouse.
This movie shows a pup retrieval assay with a Shank2−/− mouse.
To further explore the blocking potential of Cit573, a retrieval assay was performed, that is, the ability of the peptide to compete with already CCP2-bound ACPA was tested.
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DOI: http://dx.doi.org/10.7554/eLife.04580.028 To test the role of MBONs in appetitive ethanol memory, we blocked MBON function during training and memory retrieval and assayed for changes in performance.
Due to poor compliance of athletes to the third and fourth retrieval; the FORD assay was performed in all the 42 OC users and in randomly selected consecutively consenting 42 non-OC users.
We decided to use the FORT and FORD assays because, beside avoiding invasive venous blood retrieval, these point of care assays have been recently validated for use in athletes and might be used easily in sport medicine facilities [32].
Immediately after the maternal behavior assay, the pup retrieval test was conducted for a total duration of 15 minutes.
Primary EMMPRIN antibody (Zymed Laboratories , Inc San Francisco, CA; 1 μg/ml) was used on deparaffinized and rehydrated sections of the type I collagen invasion assay without antigen retrieval.
The maternal behavior assay and pup retrieval test were performed between the hours 2100 and 2300, and given the high mortality rate of pups born by dnFGFR mothers (see Results section), were only performed within 24 hours of birth (PD0).
To validate the role of these proteins in endosome-to-Golgi retrieval, the anti-CD8 uptake assay was repeated using the individual siRNA sequences of the ON TARGETplus pool in four separate siRNA transfections for 20 selected genes.
Challenges that the rover must be able to complete include a terrain traversal, a soil sampling and biological assaying, an object retrieval, panel maintenance, and an autonomous drive task.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com