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The retention volume was similar in both cases.
In addition, the values of the retention volume of hEGF under the given conditions were determined.
Two radiometabolites, less lipophilic than 11C-LY2428703, were detected with retention volume at 3.2 ± 0.7 mL and 5.2 ± 1.7 mL while the parent's retention volume was 7.2 ± 1.6 mL (n = 120).
The retention volume of the single peak on the column of Superose 6 (GE healthcare) is 11.4 mL.
In comparison to pure poly aspartic acid), this demonstrates a nearly 3-fold increase in retention volume at basic pH.
An exponential increase of the retention volume as a function of the number of arms has been found.
Similar(6)
Sometimes retention volumes, rather than retention times, are used for qualitative analysis.
However, conventional study designs may not always be appropriate to unequivocally dissociate the surface area/activity-related acute adversity from the cumulative retention volume-related adversity.
This allows predicting accurate retention volumes based on a minimum number of initial experiments.
The retention volumes were 86.46, 84.76, and 83.89 mL for Uch37ΔHb,Hc,KEKE, Uch37ΔHc,KEKE, and Uch37ΔKEKE, respectively (Fig. 4B).
It is found that three simple yet rationally chosen chromatographic experiments suffice to extract the analyte specific model parameters necessary to calculate the retention volumes.
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