Sentence examples for retention time lists from inspiring English sources
The mass and retention time lists were used for searches against the ChemSpider database, employing the in-house developed database search tool GoBioSpace (http://gmd.mpimp-golm.mpg.de/webservices/wsGoBioSpace.asmx).asmx
These values and associated retention times are listed in Supplementary Table 1.
In order to investigate the accuracy of the detected chromatographic peaks in terms of peak area and retention time, all 18 peak lists generated from the spiked-in data were aligned using algorithm reported previously, where the chromatographic peaks generated by the same metabolite in different samples are recognized based on the similarity of retention time and mass of parent ions.
There is currently no established path to relating unknowns from different studies, but listing retention time and m/ z only clearly is not sufficient.
Mass spectrometry data was analysed manually using Qual Browser version 2.0.7 (Thermo Fischer Scientific Inc .. Peak lists of different retention time segments were extracted and possible hits of lipids were identified by applying tools of the lipid maps database (http://www.lipidmaps.org).org
The standard deviation and %RSD of peak area and retention time (R t) was calculated and listed in Table?5.
A tabulated metabolite feature list with aligned retention time and m/ z values was exported in.csv format.
Preferred ions are only analyzed if they are present at the proper retention time, so extraneous ions in the list will not detract from the analysis.
Our mass defect filter removes unwanted noise from LC-MS metabolomics datasets, while retaining true metabolites, and requires only a list of m/z and retention time values.
After the first LC-MS/MS analysis, the identified peptide ions and their retention time were added to generate an exclusion list for further iterations in LC-MS/MS analysis.
Once peptides were identified by Mascot and MassMatrix and manually confirmed using retention time analysis and spectra annotation, a transition list was created from abundant fragment ions, which was used for MRM.
