Furthermore, the retention of immunoreactivity of the stably expressed L163D mutant suggests that the disarming mechanism prefers dimeric to monomeric Smac.
The scaffold/matrix attachment region (S/MAR) located 5′ of the human β-interferon gene (IFNB1) has been shown to confer a stable episomal replication and retention function within plasmid vectors when stably transfected and selected in mammalian cells.
When the protein was stably expressed in 293 cells, HIVΔVpu virion retention by tetherin R19H failed to mediate NF-κB activation.
Interestingly, this persistent retention of H3K4me in germ cells also correlates with stably increased expression of the transgene in somatic lineages in the later generations [ 14].
Meanwhile, the results of continuous cultures operated at 36 h HRT (hydraulic retention time) show that the high phototrophic H2 production efficiency was stably maintained for over 17 days with a steady-state vH2 and YH2 of 44.0 ml/h/l and 3.57 mol H2/mol acetate, respectively.
Intramyocardial injection of an FDG-containing cell lysate resulted in low retention of injected radioactivity, and ex vivo studies showed that FDG is stably retained by cells after labeling and thorough rinsing [ 27].
Interestingly, during the establishment of C33A cell lines that stably express extended-C/EBPα, the percentage of cells with nucleolar retention continuously raises up to 100%, in long-term cultures, suggesting that these cells have a selective proliferation or survival advantage (data not shown).
Indeed, we have preliminary evidence demonstrating that ER retention further increases the accumulation of some AChE variants (but not others) in stably transformed plants to about 1% of total soluble protein (Geyer, Fletcher and Mor, unpublished).
Functional BCRP was measured using BODIPY-prazosin (BODIPY) retention and Fumitremorgin C (FTC) [ 41] as a modulator with only the stably transfected BCRP OCI-AML6.2 (p = 0.015) cell line showing any functional BCRP activity.
Thus, the IKKε Spn-F complex stably localIKKε Spn-Fe distal tip by polarized transport followed by selecomplexetention, whereastably1-positive recyclocalizessomes bidirectoonally shuthee by polarizedistalsportipnd motor switching.
