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Selective retention of phosphorylated peptides is demonstrated.
Therefore, besides nuclear translocation, nuclear retention of phosphorylated Stat is also crucial for its activation.
The mechanism of cell surface delivery and retention of phosphorylated AQP2 is poorly understood.
It has been reported that phosphorylated YAP binds to 14-3-3 14-3-3 14-3-3 whinh leads to cytoplasmic retention of phosphorylated YAP.
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Second, that at a pH higher than 5, retention of singly phosphorylated peptides is greater on the SAX material than on the WAX material.
Altered cytokine production correlated with an increase in phosphorylated GSK3β, increased retention of the phosphorylated NFκB p65 subunit in the cytoplasm, and an enhanced interaction between CBP and phospho-CREB.
(E) MS1 intensity ratio (IR) of phosphorylated Atg18 peptides in nutrient-rich and starvation conditions.
ERβ decreased levels of phosphorylated HER3.
Full retention of the phosphorylated product on the filters was confirmed by running quenched reaction samples on SDS-PAGE and counting the bands.
It might be a valuable alternative to SCX, with improved fractionation and better retention of multiply phosphorylated peptides, and so it potentially could allow a more comprehensive analysis of the phosphoproteome.
YAP phosphorylation on Ser127 increases 14-3-3 14-3-3 14-3-3P, which results in the cytoplasmic retention of inactive Ser127-phosphorylated YAP [ 9, 29, 94].
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