This test, which evaluates the vesicles of the endocytic compartment by lysosomal retention of neutral red, was performed according to [ 35].
In the vehicle-treated groups, ATP13A2−/− cells consistently showed a 20 40% lower retention of Neutral red compared with the control (Fig. 1).
Neutral red is a weakly cationic dye and retained in the lysosomes depending on their pH (33) and the lower retention of Neutral red detected under vehicle treatment reflected a higher lysosomal pH in ATP13A2−/− KRS-patient cells (8, 14).
Since not many papers in the literature were dealing with the estimation of factor influence on the retention behavior of neutral and acidic compounds on aminopropyl column in HILIC, Box Behnken design and Response Surface Methodology were applied.
A combined extraction and clean-up procedure based on SPE was tested using an Oasis MCX cartridge, which is a polymeric mixed-mode cation-exchange reversed-phase sorbent enabling the retention of acation-exchange reversed-phasey, basication-exchange reversed-phase
Our aim was to generate a measure of individual differences in the ability to pursue a primary goal of short-term retention of affectively neutral words in the face of a requirement to simultaneously process emotionally-relevant sentences.
Within-group comparisons further confirmed this lack of difference between the 4 h and 15 min offline retention periods for neutral memory in the Nap group (paired t-test, t(14) = 0.22, P = 0.83) and No-Nap group (paired t-test, t(15) = 0.03, P = 0.97).
Almost without exception, traditional complex span tasks require short-term retention of emotionally-neutral information in the face of demands to process competing emotionally-neutral information (Conway et al., 2005; Schmeichel, Volokhov, & Demaree, 2008).
Neutral red retention time.
Cytotoxicity of TCC assessed in the neutral red retention assay with RTL-W1 cells.
