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In our experiments, neither L2-b nor FL2-b were found to exhibit retention in normal control tissue or high white matter uptake observed with PiB.
Next, we investigated whether the engineered bacteria with SAs and lacking natural adhesins, in addition to the improved colonization of target tumors shown previously, could have a lower retention in normal organs compared to wild type E. coli.
In contrast, there was a strong bias towards increased intron retention in normal breast versus other control tissues, wherein normal breast exhibited more frequent intron retention than any other control tissue except for endometrium (Fig. 2e).
Importantly, in vivo experiments showed that low doses of the engineered bacteria colonize solid tumors expressing the target molecule with higher efficiency than the wild type E. coli strain, having also lower retention in normal organs.
Although it has not been possible to measure individual polyglutamated forms of ZD1694, the radioimmunoassay provides a convenient means of assessing total drug levels in tissues and is currently the only method suitable for measuring the extent of drug retention in normal tissue and tumour biopsies obtained from patients treated with ZD1694.
Median gene expression of MKI67, encoding the proliferation marker Ki-67, was >10-fold higher in breast cancer samples than in adjacent controls, indicating that enhanced intron retention in normal breast is unlikely to be explained by contamination of adjacent control tissue by cancerous cells.
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The intervention helped women meet their postpartum weight goals by reducing postpartum weight retention in normal-weight women and helping overweight or obese women to lose weight.
Recently presented data on three Roche tau tracers in humans showed that 18F-RO6958948 has a promising clinical profile, with good brain uptake and little retention in cognitively normal young individuals (Wong et al. 2015).
Consistent with this, others have reported that blocking the interaction between the Stat3 target gene Icam-1 and lymphocytes decreased lymphocyte retention in the normal pulmonary vasculature (Klemm et al, 2000).
Water retention occurs physiologically in normal pregnancy.
Systemic administration of angiotensin II caused a significant retention of marker in normal liver, but not in tumour compared to controls.
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