Suggestions(1)
Exact(8)
The bound complex could be competed by an unrelated CTCF binding site (F1), whereas the negative control, GST protein expressed in E.coli, resulted in no specifically retarded complex.
This retarded complex formed with the MMP-1 probe was completely abolished by excess unlabeled wild-type AP-1 probe (Fig. 3C, lane 3).
Supershift assays indicated that the observed retarded complex corresponded to the p50/p65 heterodimer (data not shown).
As shown in Figure 2B, incubation of the AP-1#1 probe with nuclear extracts from mock-treated HeLa cells resulted in the formation of a broad retarded complex designated AP-1 1-C1 AP-1 1-C1 AP-1 1-C1, lane 1).
The single retarded complex detected was efficiently competed by an excess of unlabeled probe (Fig. 6A, lanes 4 and 6) and partially supershifted upon addition of an anti-C/EBPα antibody (Fig. 6A, right panel), which attested its specificity.
On the other hand HupBMtbN while retaining its ability to interact more proficiently with AT rich DNA, forming a single gel retarded complex (Figure 5B, lanes 9 14), displayed weak interaction with GC rich DNA (Figure 5B, lanes 2 7).
Similar(52)
Results indicated that the PPy sheath played a role of inhibitor and retarded the complex chemical reactions of PAN during the carbonization process.
However, it is also possible that disordered regions of the protein retard complex mobility during size-exclusion chromatography.
It was notable by EMSA using recombinant eEF1α that the complex formed was retarded less than the complex formed between the MT-111 RNA and CHO cell lysate (see Fig. 3D), suggesting that proteins other than eEF1α in the CHO cell lysate may also be involved in complex formation.
The experimental construction and the DPV detection were based on the fact that the formed antibody antigen immune complexes retarded the electron transfer tunnel of gold electrodes.
Both bands were significantly shifted whereas the higher bent complex was retarded (lane 4) with respect to the more linear adduct (lane 5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com