These observations suggest that CD4+ cells surviving the expansion phase of immune response produce a Th1-signature cytokine and retain responsiveness to IL-4, a Th-2 cytokine, as well as to a well described survival factor, interleukin-7.
MLTCs are also known to retain responsiveness to human chorionic gonadotropin (hCG) [6] [8].
We also hoped to retain responsiveness of these constructs to α-factor.
Transfection of these constructs revealed that they retained responsiveness to exogenous VDR in CaCo2 cells and VDRK240−/− (Figure 5B and Supplementary Figure S4B, respectively).
Cells derived from human pancreatic ducts were initially expanded as a primary culture and only early passage cells that retained responsiveness to secretin stimulation were used [11].
Immunodetection of the validated protein band for threonine 287-phosphorylated CaMKII demonstrated that the introduced β CaMKII retained responsiveness for contraction-induced CaMKII signalling.
To determine if neoplastic cells retained responsiveness to CSC, we measured the effects of CSC on FANCD2 expression in bronchogenic carcinoma cells (A549 and H292).
However, Nanog null ESCs retain responsiveness to Gsk3 inhibition (Silva et al., 2009), and knockdown of Nanog in Tcf3 null ESCs does not prevent upregulated expression of other pluripotency genes (Yi et al., 2011).
Notably, the whole sets of 5-aza-dC targets and RA targets retained responsiveness to CBP, as indicated by the corresponding Pearson correlation coefficients of 0.66 (middle panel) and 0.78 (right panel), respectively.
