Similarly, IST1R 4SA retained its interactions with all known IST1-binding partners in Y2H assays.
The mutant lacks a DNA-binding domain, yet retains its protein-interaction domain, thus we hypothesize that the mutant may sequester crucial transcriptional partners to block expression of gene targets, similar to the Inhibitor of Differentiation proteins such as Id2 [ 73, 75].
However, we found no overt mislocalization of membrane-bound E-cadherin in cultures of intact colonic crypts isolated from Bcat mice, consistent with our biochemical analysis and the prediction that ΔN-Bcat protein retains its E-cadherin interaction domain (Fig. 2A,B; Fig. S1I).
10.7554/eLife.06421.009 Figure 5. Full-length MCAK remains dimeric upon disruption of the motor-CT tail interaction but retains its depolymerase activity.
The interactions between the biomolecule and the inorganic, organic or hybrid nanocomposite material determines the degree to which the biomolecule retains its native properties, and such interactions can be tuned to provide optimised biomaterials that are suitable for a variety of applications.
For example, aggrecan, the major proteoglycan in cartilage, is retained through its interaction with HA, forming large aggregates of aggrecan along a HA backbone and enabling cartilage to resist mechanical loads.
Each MNT module retained its intended function: high affinity interaction with EGFR and α/β-importin dimers, ensuring nuclear transport of NLS-containing protein, formation of holes in lipid bilayers at endosomal pH, and accumulation in the nuclei of A431 cells [5].
Observation from western blot profile of disrupted sample indicates that HBsAg recovered from the cell disruption process did not suffered from any noticeable damage or degradation and had retained its antigenic properties in generating interaction towards monoclonal anti-HBsAg.
Secondly, we excluded the data set by Collins et al. (30) from Phase II and retained all its interactions as binary undirected.
Importantly, the presence of benzonase during cell lysis eliminated all DnaN from the plug, indicating that circular DnaN is retained in plugs via its interaction with cellular DNA.
