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A small GroEL-binding tag made it possible to perform all steps of the encapsulation under near physiological conditions while retaining the native conformation of the target protein.
By retaining the native distal His64 in sperm whale myoglobin (Mb), a second distal histidine was engineered in Mb by mutating Leu29 to His29.
The native amino acid sequence was reverse translated and the resulting nucleotide combinations manipulated in order to evenly distribute 25 unique restriction sites along the length of the gene while retaining the native amino acid sequence.
In this study we propose that M2 protein can be used as a molecular fabricator without disrupting the assembly of VLPs and while retaining the native structures of HA and NA envelope protein oligomers on the particle surfaces.
On the other hand, there is no region retaining the native β-sheet secondary structure.
The challenges are clear for retaining the native configuration and phenotype of important cell types along with the local hemodynamic conditions observed in vivo.
Similar(51)
The spectroscopic results revealed that the immobilized Hb retained the native catalytic activity and conformation.
Ultraviolet-visible and Fourier transform infrared spectroscopic results indicated that Hb molecules in the composite film retained the native structure.
The matrix is derived from porcine dermis, and is processed to retain the native collagen (Type 1) and elastin structure.
The challenging joint-preserving surgery that retains the native joint with an optimal function may benefit from these assistances.
BAC transgenes usually retain the native gene architecture including all cis-regulatory elements as well as the exon intron configurations.
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