Exact(4)
Neural precursors were mechanically isolated and cultured in suspension as neurospheres, which extinguished the expression of pluripotent factors NANOG, LIN28 and OCT4 while retaining the expression of SOX2, a transcription factor expressed in neuronal precursors (Fig. 2B).
Polyclonal CAR+ γδ T cells have been successfully generated retaining the expression of receptors displaying inherent antitumor activity [ 43, 44].
To reduce noise, we adapted the approach of Iorio et al. (2010) for our setting, by retaining the expression of top 2000 up- and 2000 downregulated genes for each sample, while considering the rest as noise (set to zero).
Long-term cultured spermatogonia were here shown to be a heterogeneous population of cells, although still retaining the expression of several genes of pluripotency (especially LIN28, SOX2, and NANOG).
Similar(56)
Although we see that the cell lines retain the expression of most genes, the differentially expressed genes between tumors and cell lines are very important to investigate while we translate our discovery from in vitro to in vivo.
FF-hESCs retained the expression of the pluripotent marker Oct-4, a POU domain transcription factor exclusively expressed in undifferentiated hESCs (Figure 1A).
Then, let us retain the expression of (alpha_{1}) and introduce the following error: e_{2} = hat{z}_{3} - alpha_{1},.
Co-cultures on D-PHI released more IL-10 (anti-inflammatory) than monocytes cultured alone, while the VSMCs retained the expression of its marker protein calponin.
Thus CD146hi stromal cells from primary cultures retain the expression profile associated with the HSC niche.
Monocytes stained with the lectins wheat germ agglutinin (WGA) and peanut agglutinin (PNA), but not soybean agglutinin (SBA), whereas macrophages and fibrocytes retained the expression of WGA, but did not stain with PNA or SBA (data not shown).
Flow cytometric analysis disclosed that 20 40% of expanded human VPC-derived EC retained the expression of the endothelial cell-related markers, including VE-cadherin, VEGFR2, CD34, CD31 and CD105, and all of the cells were negative for a panleukocyte marker CD45, monocyte/macrophage marker (CD11b), and stem/progenitor makers (AC133 and c-kit) (Figure 1a).
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