When grown under either the low or high osmolarity regimes, osmosensing circuits within EPB30/pRD400 cells could tolerate a roughly 10-fold range in EnvZ-V5 levels while retaining steady-state signal output similar to MDG147/pEB5 cells.
To retain steady growth numbers, production costs were often over-reported when they did not meet state guidance, and vice versa when excess was produced, creating inaccurate statistics and error-laden financial reports.
Two point mutants, coq5- 2 (CH83-B3) and coq5- 5 CH316-6BB) laC-methyltransferaseactivityvity and Q6 biosynthesis but retain steady state levels of Coq5 and other Coq polypeptides [15,16,29].
Previous work in yeast has shown that certain point mutations within or adjacent to conserved COQ5 methyltransferase motifs result in a loss of Coq5 methyltransferase function, but mutants harboring these alleles (coq5- 2, coq5- 5) still retain steady state levels of Coq5 protein [15,16].
It's tempting, if perhaps intrusive, to wonder whether at some level Begley has retained steadier belief in himself as lawyer than as writer.
With just over four weeks until the referendum, the "Remain" side is retaining a steady lead in opinion polls—seven percentage points in the poll of polls in Financial Times and British bookies are offering odds of around 4 1 on a Leave vote, against 1 6 for remaining.
They did note, however, that there can be a fair amount of work gaining new clients and retaining a steady clientele.
Calculations show that grain boundaries with size of 200 nm can rotate by ∼30° during ECAP, thereby generating and retaining a steady-state equiaxed structure.
Davis said: "My objective is to retain this steady growth so when he joins he can take us on our next step forward".
If winning is an acquired habit so too is losing, and no batsman at any stage looked like going on, batting through, or taking the game away from Australia's bowlers, who retained a steady hand on the scruff throughout.
Heavy with a sense of melancholy conveyed through a gently luminous colouristic range, the Love Scene retained a steady momentum, each of its successive climaxes carefully measured and positioned within the overall scheme.
