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Plasmid pPH1JI was then removed by conjugating pLAFR1 into all strains, and selecting for loss of the gentamicin marker while retaining resistance to kanamycin and tetracycline.
Individual clones were expanded and screened for physical linkage between pur and PTRE-HPRT by identifying clones with low frequency PTRE-HPRT inactivation (via TG selection) while retaining resistance to puromycin.
Recently, the multiple functions of TNF-α in granuloma formation and control of infection and disease pathogenesis were addressed using a computer-generated model, which predicted that a critical balance of TNF-α bioavailability must be maintained in order to achieve reduction of inflammation while retaining resistance to infection and microbial disease [54].
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Similarly we have observed retained resistance for the remaining resistant cell lines after culture for more that 15 weeks without drug.
As expected, the bands were bright as the GBMs retained resistance.
For additional rounds of backcrossing, the progeny that retained resistance were crossed, depending on their mating type, with either SAK84 or SAK690.
The opr3 mutant retains resistance against Bradysia impatiens and Alternaria brassicicola [ 58, 59] and partial resistance against Sclerotinia sclerotiorum [ 60], suggesting that JA/JA-Ile is dispensable as a defense signal and may be substituted by OPDA.
In particular, when Car-R males were crossed with inbred SWR/J females, which are susceptible to skin and lung cancer, their F1 progeny retained resistance to both tumors, measured quantitatively as reduced incidence and lower tumor multiplicity.
SU11274 resistant MU and RU cells (MU-R, RU-R) were cultured in the absence of SU11274 for 12 passages, passed on a weekly basis, and found to retain resistance.
Some species in this genus have retained resistance to sub-zero temperatures even if they do not experience them in their current habitat, with the freezing temperature (also measured as the release of latent heat at the supercooling point) between −6°C and −8°C in species living at different depths in the cave [ 47].
Composites fabricated with Al2O3 particles exhibit the highest fracture resistance approaching 60 MPa m1/2, while laminates prepared from Al2O3 platelets exhibit higher strengths (above 700 MPa) while retaining fracture resistance up to ∼40 MPa m1/2.
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