Total RNA was isolated using a small RNA-retaining protocol that employed an organic extraction followed by glass-fiber immobilization (Ambion, TX).
It will therefore require internet providers to retain Internet Protocol - or IP - address data to identify individual users of internet services.
Internet providers will have to retain Internet Protocol address data to identify individual users.
The Home Secretary, Theresa May, has said the legislation would "require internet providers to retain internet protocol - or IP - address data to identify individual users of internet services".
Total RNA was isolated from ~5×10 cells using the mirVana™ miRNA isolation kit (Ambion) according to the manufacturer's protocol (retaining the small RNA fraction in the pool of total RNA).
In addition, its advantage over the legacy method lies in that the latter can just replace the old authentication protocol with a new one while our proposal can add a new protocol while retaining the existing ones, which can realize forward compatibility and enables the terminal and the operator to negotiate and choose a suitable protocol.
We will follow our protocol for retaining subjects in the study very closely in order to reduce attrition.
In addition, the protocol retains a five-year period during which any successful challenge to an original trademark application would nullify all international trademarks.
Following addition of beads, they are retained throughout the protocol until the post-adaptor ligation size selection step.
This protocol retains specificity at subfamily-defining regions or motifs, while generalizing well to more distant homologs.
Despite these technical achievements, the standard Vibratome sectioning protocol retains two important disadvantages: the actual sectioning procedure is very time consuming and some tissues are particularly difficult to cut.
