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It was found that the molecular weight distribution of the isotactic polymer could be controlled while retaining high activity, high isospecificity and high molecular weight only by changing the kind and/or amount of the Lewis bases.
Almost 10% of S. pneumoniae isolated were resistant to penicillin, macrolides, and at least one of the other antibiotics tested, with only telithromycin and levofloxacin retaining high activity against these isolates (99.0% and 98.1%, respectively).
Kermekchiev and colleagues randomly mutated the large (5′-to-3′ exonuclease-deleted) fragment of Taq DNA polymerase (Klenow fragment of Taq DNA polymerase, or KlenTaq1) and screened mutants for low activity at 37 °C while retaining high activity at 68 °C.
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More recent developments include the Magnacat™ process for selectively retaining higher activity catalyst and process additive chemicals for enhancing catalyst performance.
At doses less than, or equivalent to, the LD10, isophosphoramide mustard retained high activity against cyclophosphamide-resistant L1210 and P388 leukaemias, but was less active against intracerebrally-implanted P388 leukaemia while cyclophosphamide produced a 4 log10 tumour cell reduction.
In the presence of organic solvents, detergents and different concentrations of NaCl, EstA retained high activity.
This mutant also showed a high increase in temperature stability (Tm was raised by 13 °C) and retained high activity towards cephalosporin C under standard assay conditions.
In contrast to other previously characterized cholesterol oxidases, this enzyme retains high activity in organic solvents and detergents at temperatures above 85 °C despite its mesophilic origin.
Most of the mutants still retained high activity on β-carotene, however, the F213L single mutant and the R203W/F213L double mutant that yielded the highest improvement for astaxanthin production showed decreased activity for canthaxanthin production.
Owing to this difference, moxifloxacin may retain high activity against increasingly common pneumococcal strains bearing substitutions in topoisomerase IV due to the wide use of old derivatives.
The transposases encoded by foreign genes have retained high activity, indicating a continuous exchange of 7653R and MAFF303099 genetic information with other species.
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