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Filtering for retaining clusters used the following count restrictions Cf > = 1, Gm > = 2, Mt > = 1, Vv > = 1, Cf+Gm+Mt+Vv < = 100.
To detect these regions of higher divergence systematically, we clustered 1-kb windows with >1.4% bases different or >500 bases deleted or inserted, retaining clusters of ≥9 kb.
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Non-redundant (NR) transcript sets were created by clustering sequences with cd-hit-est version 4.6 [ 46] with sequence identity threshold set to 1.0 and retaining cluster representatives (the longest sequences).
It was then checked that the number of retained clusters is realistic with respect to the field observation in order to be accepted as a meaningful classification.
All retained clusters were partitioned into SV classes (deletions, inversions, and transpositions) based on the paired read configuration.
Taxonomy was assigned to the retained clusters (OTUs) based on the Greengenes reference sequence and the Greengenes tree was used for all downstream phylogenetic community comparisons.
We retained clusters with a threshold of p < 0.001 uncorrected at the voxel level, and a corrected threshold of p < 0.05 at the cluster level after SPM standard correction for multiple comparisons.
After that, all the aligned sequences were clustered and we only retained clusters that had exactly one representative member from each subgenome and located on similar position of homeologous group.
It was made evident that WGD is often followed by a genome-wide process of biased fractionation that preferentially targets one sub-genome to retain clusters of dose-sensitive genes often organized in functional modules [ 57– 57].
It is known that polyploidy is succeeded by a genome-wide process of biased fractionation, preferentially targeting one subgenome to retain clusters of dose-sensitive genes organized in functional modules (Thomas et al. 2006).
At the five hour time point, 50% of cells had not entered meiosis (50% of the cells retained clustered centromeres and were negative for Zip1 staining).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com