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Introduction of spatial patterning of proteins, while retaining activity and releasability, is critical for the field of regenerative medicine.
This series represents a significant improvement on previous classes of compounds, being substantially more potent and largely retaining activity in the presence of serum.
We previously developed "self-assembly PEGylation retaining activity (SPRA) technology" via a host guest interaction between PEGylated β-cyclodextrin and adamantane-appended insulin, and resulting PEGylated insulin was termed SPRA-insulin.
And it is a well-known fact that the 5-position of the tryptamine molecule accepts a wide variety of substituents while retaining activity. .
In six steps, we eliminated a common buried residue, Phe, from the green fluorescent protein (GFP), while retaining activity.
These studies have clearly demonstrated role of formulation in retaining activity of biologics.
Similar(43)
Then, the retaining activities were assayed using 1 mM p-NP-C4 as substrate.
Highly active compounds also retained activity against H. capsulatum phagocytosed into P388D1 macrophages.
These compounds are active against wild type reverse transcriptase (RT) and retain activity against clinically important mutants.
Clearly, because a protein is active in a cultured cell assay, it cannot be assumed to retain activity in vivo.
The paclitaxel-loaded nanoparticles retained activity in cell lines in vitro.
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CEO of Professional Science Editing for Scientists @ prosciediting.com