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Additionally, lines were karyotypically normal after multiple passages and have been propagated for over 30 passages in culture while retaining a normal karyotype (Figure S3).
In human patients, a fast channel myasthenic syndrome is caused by a P121L mutation in the AChRε subunit, reducing the postsynaptic response to ACh while retaining a normal AChR load on the endplates (Ohno et al, 1996).
Furthermore, the tissue changes we noted were not generalized but focal, with certain parts of the muscles retaining a normal morphology (c.f. Figure 1).
In healthy humans, serum K+ levels are tightly controlled within the narrow range of 3.5 to 5.0 mEq/L [ 2], thus retaining a normal ratio between the intracellular and extracellular compartments.
Mean PEG treatment was for 15.3 months; all patients normalised IGF-1 and 5 were able to reduce frequency of dose administration to less than daily while retaining a normal IGF-1.
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Patients affected with isolated glucocorticoid deficiency, a hallmark of hypopituitarism, usually retain a normal volemic status, in contrast to patients with primary adrenal insufficiency.
The other cell line, H9, retained a normal XX karyotype after 6 months of culture and has now been passaged continuously for more than 8 months (32 passages).
One of the first lessons gleaned from these large-scale studies is that hESCs are commonly diploid soon after derivation and can retain a normal karyotype after many passages (for example, in the ISCI study 66% of lines remained normal).
Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype.
In the mitochondrion of LD-CMS, there is only one copy of atp6 linked with orf79, which is different from BT-CMS and HL-CMS, the mitochondria of which retain a normal atp6 (N-atp6) in its origin position (Itabashi et al. [2009]).
As highlighted in the following sections, most of the issues derived from it can be solved at the patient's bedside, the great bulk of patients belong to the subgroup exhibiting hypotonic hyponatremia and those with ill-tolerated hyponatremia either retain a normal ECFV or display a diminished ECFV.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com