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The retained transcript of the intron region in most cases encodes amino acids in-frame with the neighboring exons [ 19].
While canonical Csnk2b isoform was widely expressed, Ly6g5b canonical isoform was less ubiquitous, although the Ly6g5b first intron retained transcript was present in all the tissues and species analysed.
After the first filtering, the degradome signals along each retained transcript were obtained from the BLAST results to provide a global view of the signal noise when compared to the signal intensity within a specific target binding site.
The resulting retained transcript sets containing transcripts above the abundance threshold and containing a likely open reading frame were merged and subjected to annotation and utilized in subsequent analysis.
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These retained transcripts were subjected to a second BLAST, and the degradome signals along each full-length gene were obtained to provide a global view of the signal noise when compared to the signal intensity within a specific binding site.
Similar to a previous study, SHSs on the boundary sites of these retained transcripts were checked.
We generally only retained transcripts with full-length coding sequences (but see below).
This resulted in 47,542 retained transcripts, which we designate as the unfiltered set.
Thereafter, the retained transcripts are called 'driver transcripts' and modules that contain at least one driver are termed 'GEMOT modules'.
The retained transcripts from the Trinity assembly also included representation of 32% more of the transcripts in the Ensembl zebrafish database.
Though rare examples exist for nuclearly retained transcripts (Prasanth et al., 2005), a global picture of mRNA nuclear retention in mammalian tissues is lacking.
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