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The upscaled WJ-MSCs retained their differentiation potential and immunosuppressive capacity.
To overcome this problem, we attempted to prolong the life span of human placenta-derived mesenchymal cells (hPDMCs) by modifying hTERT and Bmi-1, and investigated whether these modified hPDMCs retained their differentiation capability and multipotency.
We demonstrated that young MSCs retained their differentiation potential (measuring adipogenic differentiation; data not shown), but that HBO had no effect on this process.
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However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage.
Placing the inner cell mass in vitro under the finely tuned culture conditions prevents their imminent differentiation and allows cells to self-renew seemingly indefinitely whilst retaining their differentiation potential (Thomson et al., 1998).
Alternative differentiation is observed in human keratinocytes lacking p16INK-4a, expressing telomerase and retaining their differentiation capacities (N-hTERT cells) upon sublethal repeated exposures to UVB [7].
Repeated exposures to sublethal doses of UVB induce an alternative differentiation state rather than premature senescence in cultivated human keratinocytes lacking functional p16INK-4a, immortalized with telomerase and retaining their differentiation capacities (called N-hTERT cells herein) [7], [8].
Mad was strongly expressed in differentiating suprabasal keratinocytes in healing dermal wounds and in benign hyperproliferative conditions, but also in squamous cell carcinomas, in which the keratinocytes retain their differentiation potential.
The cells retained their adipogenic differentiation capacity inside the gel, as verified by lipid accumulation.
This approach resulted in homogeneous structures in which cells were viable and retained their osteoblastic differentiation potential.
Upregulation of the early lineage-specific genes PPARγ, LPL, and COMP was observed in the ASCs expanded on the DAT microcarriers, but the cells retained their multilineage differentiation capacity.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com