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The spectroscopic results revealed that the immobilized Hb retained the native catalytic activity and conformation.
Ultraviolet-visible and Fourier transform infrared spectroscopic results indicated that Hb molecules in the composite film retained the native structure.
Although the fragment retained the native conformation and affinity to a protective antibody, its vaccine efficacy and conformational stability were significantly reduced with respect to full-length OspA.
To account for this possibility, we created a second mutagenic construct that retained the native aphA-3 promoter30, and used it to re-mutagenize the 142 genes that had generated no recoverable mutants (Figures S1).
We therefore co-expressed combinations of full-length wt RNF4 or its SIM mutants with poly-SUMO chains that retained the native N-terminus on the first SUMO moiety and were C-terminally tagged with GFP HA in S. cerevisiae.
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The matrix is derived from porcine dermis, and is processed to retain the native collagen (Type 1) and elastin structure.
The challenging joint-preserving surgery that retains the native joint with an optimal function may benefit from these assistances.
BAC transgenes usually retain the native gene architecture including all cis-regulatory elements as well as the exon intron configurations.
A small GroEL-binding tag made it possible to perform all steps of the encapsulation under near physiological conditions while retaining the native conformation of the target protein.
By retaining the native distal His64 in sperm whale myoglobin (Mb), a second distal histidine was engineered in Mb by mutating Leu29 to His29.
Our results show that the fusion protein is a monomer in aqueous solution, forms a stable intramolecular complex, and likely retains the native conformation of the complex between Bcl-xL and the HBx BH3-like motif.
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