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We have retained the label "CC", however, since we are confident of the assignment.
Individual rat kidney tubule cells were shown to incorporate BrdU and retained the label during a short (2 week) chase [ 130].
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Cuba retains the label of "rogue state" and supporter of terrorism, while the US refuses to allow Luís Carriles Posada to be extradited.
For any fashionable purveyor of consumer goods, be it a rock band, a clothing line, or a cell-phone manufacturer, retaining the label of "cool" is the hardest thing of all.
Cells that are not replicating retain the label.
One characteristic of bulge stem cells is that if they incorporate BrdU in neonatal epidermis they retain the label in adulthood; such cells are known as label retaining cells (LRC) [21], [22].
Within this category of testicular cells, somatic cells were found (e.g. Sertoli and interstitial cells), but type A undifferentiated spermatogonia (Aund* and Aund) were the only germ cells able to retain the label after 18 and 25 days of chase, constituting therefore the putative SSCs.
Cells that are quiescent and therefore have not divided retain the label and can then be isolated for further assay.
One characteristic of bulge stem cells is that if they incorporate BrdU in neonatal epidermis they retain the label in adulthood; such cells are known as label-retaining cells (LRCs; Bickenbach and Chism, 1998; Braun et al, 2003).
Therefore, we sought to develop a general methodology for labelling PPMOs that utilizes commercially available, unfunctionalized PMOs and which would retain the label close to the PMO in the event of peptide proteolysis in vivo or intracellularly.
This is, however, not without problems (e.g. differentiated non-proliferative cells are likely to equally retain the label) and reliable and specific markers of stemness are urgently sought.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com