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FF-hESCs retained the expression of the pluripotent marker Oct-4, a POU domain transcription factor exclusively expressed in undifferentiated hESCs (Figure 1A).
Co-cultures on D-PHI released more IL-10 (anti-inflammatory) than monocytes cultured alone, while the VSMCs retained the expression of its marker protein calponin.
Monocytes stained with the lectins wheat germ agglutinin (WGA) and peanut agglutinin (PNA), but not soybean agglutinin (SBA), whereas macrophages and fibrocytes retained the expression of WGA, but did not stain with PNA or SBA (data not shown).
Flow cytometric analysis disclosed that 20 40% of expanded human VPC-derived EC retained the expression of the endothelial cell-related markers, including VE-cadherin, VEGFR2, CD34, CD31 and CD105, and all of the cells were negative for a panleukocyte marker CD45, monocyte/macrophage marker (CD11b), and stem/progenitor makers (AC133 and c-kit) (Figure 1a).
In mixed GCT each component retained the expression characteristics consistent with its microscopic appearance (Table 1).
They retained the expression of SP-C, CCSP and CD34 mRNAs over time.
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Neural precursors were mechanically isolated and cultured in suspension as neurospheres, which extinguished the expression of pluripotent factors NANOG, LIN28 and OCT4 while retaining the expression of SOX2, a transcription factor expressed in neuronal precursors (Fig. 2B).
Although we see that the cell lines retain the expression of most genes, the differentially expressed genes between tumors and cell lines are very important to investigate while we translate our discovery from in vitro to in vivo.
Then, let us retain the expression of (alpha_{1}) and introduce the following error: e_{2} = hat{z}_{3} - alpha_{1},.
Thus CD146hi stromal cells from primary cultures retain the expression profile associated with the HSC niche.
Because TGCTs and fetal germ cells retain the expression of the "ESC-specific" alkaline phosphatase enzyme visualized by NBT BCIP and Kiel, these staining protocols can be used to stain TGCTs [18], fetal gonocytes and oogonia [15], [16], and other tumours and ESC-like cells with ESC-like properties expressing alkaline phosphatase [14], [18].
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