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We had cultured H9 hES cells through multiple passages while they retained stable expression of undifferentiated markers of hES cells, and a capacity to differentiate to three germ layer cells.
Despite several attempts, we were unable to isolate lines that retained stable expression, suggesting that high expression of CCBE1 was incompatible with growth or survival (data not shown).
10 CHO and 4 PK15 clones were obtained, in which 100% of the cells retained stable expression of Sn and CD163 for at least 15 passages.
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Six retained stable GFP expression in the absence of selection (Fig. 2B).
This resulted in 1 partial response and 7 patients retained stable disease.
Interestingly, this truncated reduced the ability to bind Rpt6 and Rpt3, yet retained stable association with Rpt1 and Rpt4.
Tissue morphogenesis and organogenesis require that cells retain stable cell-cell adhesion while changing shape and moving.
Despite the expression of Syk-EGFP, the MAP1B knockdown cells displayed a morphology similar to that of cells lacking Syk as cells were more rounded and failed to retain stable microtubule networks.
The results suggest that porcine DFAT cells retain stable growth characteristic and efficient proliferative potential.
Under such conditions, stem cells can retain stable growth capacity to guarantee their sustainable differentiation.
Hypoxia-tolerant cell lines retain stable mitochondrial membrane potential and ATP concentration when incubated under oxygen limiting conditions.
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