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However, were this group to retain the flexibility observed when unbound upon binding (i.e., oscillation between two modes; see Figs. 2C and 5), the electron density map could be effectively accounted for; it is therefore proposed that the X-ray data is better accounted for by a structural model in which the flexibility observed in solution for this group is retained on binding.
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After extensive washing in the same binding buffer, proteins retained on the solid phase were eluted by the addition of SDS sample buffer and subjected to SDS-PAGE for fractionation prior to being transferred onto PVDF membrane.
Filters were washed twice with 5 ml ice-cold binding medium, and [3H]NLX retained on the filters was measured by liquid scintillation spectrometry.
Notably, transcription factor FoxA1 is globally retained on mitotic chromatin as visualized by IF, even though its binding at specific binding sites as measured by ChIP is substantially diminished [ 20].
Therefore, in this paper we used the Wien effect to evaluate the co-adsorption impacts on the binding energies of Cd2+ and Zn2+ retained on soil clay fractions.
Our design did not include the HBD region and only transcription factors with predicted binding sites in all programs were retained on the list.
After several steps of binding and washing, bound STAT3 probe is retained on the filter and the free DNA probe is removed.
Proteins retained on the column were displaced by sequential washes with cysteine, glutathione, and dimercaptopropanesulfonate (DMPS) after removing nonspecific binding materials with buffer and ethylene glycol.
The amount of SFK retained on the beads is a reflection of the relative accessibility of their SH3 domains to engage in intermolecular binding interactions.
Sprite fittings were retained on production aircraft.
Polar compounds are retained on the column.
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