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For in vitro studies, cryopreserved samples (10/10) retained normal cell architecture and vascular endothelial integrity upon H/E and factor VIII staining.
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APC activates cell signaling networks with alterations in gene expression profiles by activating protease activated receptors 1 and 3. To minimize APC-induced bleeding risk, APC variants were engineered to lack > 90% anticoagulant activity but retain normal cell signaling.
The expression of ITGB3 in tumours from survivors in our study may indicate that these tumour cells still retain normal cell properties, and are therefore less aggressive.
Puromycin-selected cardiomyocytes exhibited a gene expression profile similar to that of adult human cardiomyocytes and generated force and action potentials consistent with normal fetal cardiomyocytes, documenting these parameters in hESC-derived cardiomyocytes and validating that the selected cells retained normal differentiation and function.
Six cancer cell lines retained normal copy numbers.
Both the Zante and Cardiff cell lines have retained normal growth controls, as judged by retaining their ability to exit from the cell cycle in response to contact inhibition (data not shown).
In contrast, Medaka FADD failed to induce cell death in Casp8-deficient MEF cells, as the majority of EGFP-positive cells survived and retained normal morphology.
It appears therefore that the IPC cells have retained normal bone marrow properties like pleiotropic response to cAMP and Bim-dependent death induction.
The engineered keratinocytes retained normal morphology and grew in a manner similar to lentiviral-treated control cells.
Most of the cancer cells recovered were viable (94%) and could be cultivated for >8 days, during which period they retained their normal cell morphology and proliferation rates.
Most of the TI chronically treated cells exhibited abnormal chromosome number (Fig. 4A), with only ∼40% of cells retaining normal chromosome number.
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