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For example, there is a genomic block comprising 225 genes, which is linked to Hox cluster, that was already present in the eumetazoan ancestor and has retained linkage in both human and anemone genomes (Putnam et al. 2007).
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Because we expected seven linkage groups in both parents, we retained the linkage groups generated at a LOD of 3 for MRD60 and at a LOD of 4 for MRD30.
Only those fitting the single-locus model were retained for linkage mapping purposes.
Only SNPs that had high quality of genotype calls and were heterozygous in at least one parent were retained for linkage analysis.
The Kaiser criterion was applied [ 56] in order to retain only factors with eigenvalues greater than 1: one to three factors per trait containing the overwhelming majority of the total variance (Table 2) were retained for linkage analyses.
After elimination of SNPs with missing data >10% or loci with significant segregation distortion from a 1 2 1 ratio as measured by χ at the 1% significant level, 6531 SNPs were retained for linkage analysis.
After further filtering to remove SNPs with low calling rate (<97%) and non-Mendelian inheritance (P < 0.001), a total of 18,866 SNPs from family 1, 33,224 SNPs from family 2, and 35,093 SNPs from family 3 were retained for linkage mapping.
We retained pathway linkages for which the number of supporting interactions was greater than that of at least 99.9% of randomized networks.
This process retains any linkage between the two markers, but randomizes them relative to all phenotypes and covariates.
Mutations further apart than 100 bps are hence often separated by recombination and retain little linkage consistent with the observed decay length in Figure 7.
One of our strategies to balance the resolution and FPF was to use both ancient LD and genetic linkage retained in the crossing process.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com